Sanger Sequencing FAQs
GENEWIZ Sanger Sequencing frequently asked questions including service and DNA type, troubleshooting, and sample submission.
Sample Storage Policy
Sample Storage Policy Please reference your service line and region below to find the relevant Sample Storage Policy at GENEWIZ from Azenta: Service GENEWIZ Lab Location Sample Storage Policy NGS All US Labs UK Lab Germany Lab *For information regarding the Sample Storage Policy for NGS samples processed in one of our China labs, please…
Clinical Services Sample Submission Guidelines
Clinical Services Sample Submission Guidelines Microarray Solutions
Cloning & Mutagenesis
Cloning and Mutagenesis Sample Submission Guidelines Please submit all required starting material (typically 5 µg of plasmid DNA) to GENEWIZ as soon as possible after initiating your order (if required). A detailed list of the required starting material is included in the “client” section of your quotation. Direct Shipping (FedEx, UPS): If a Drop Box…
Molecular Genetics
Sample submission guidelines for GENEWIZ PCR + Sanger services including nucleic acid extraction, cDNA and gDNA verification, and SNP/Mutation analysis.
Plasmid Preparation Sample Submission Guidelines
Plasmid Preparation Sample Submission Guidelines Sample Type* Sample Guideline Clonal Plasmid DNA 5 µl at 200 ng/µl (minimum 100 ng) Colonies on agar <3 days old; single and well isolated Clonal Agar stab samples agar stabs Clonal Glycerol stocks 200 µl of frozen stock Frozen bacterial cell pellets varies by scale * Only GMOs with…
Next Generation Sequencing Sample Submission Guidelines
Next Generation Sequencing is a technology in which nucleotides are added in parallel to the copying of a DNA strand. When nucleotides are incorporated into the growing DNA strand, a signal is generated that corresponds to the nucleotide type and position. GENEWIZ Next generation sequencing services offer both standard and custom options for extraction, library preparation, sequencing, and bioinformatics.
Gene Synthesis Sample Submission Guidelines
Gene Synthesis Sample Submission Guidelines Please submit all required starting material to GENEWIZ. For UK projects (40-) samples will need to be submitted to the UK subsidiary and for continental Europe projects (50-/90-) samples will need to be submitted to the German subsidiary (addresses below) as soon as possible after initiating your order (if required)….
Sanger Sequencing Sample Submission Guidelines
Follow our Sample Submission Guidelines as closely as possible for the best Sanger sequencing results.
Free Universal Primers
GENEWIZ offers a variety of free universal primers for Sanger sequencing.