Targeted Sequencing
Targeted sequencing is a technique that uses next generation sequencing to target specific genes, coding regions of the genome, or chromosomal segments for rapid identification and analysis of genetic mutations. Whether you want to pinpoint a few genes or a few hundred, our custom targeted sequencing panels offer high sensitivity and specificity, providing in-depth coverage and high-quality data to discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.
To investigate the location and frequency of vector integration in the host genome following transduction, use our integration site analysis (ISA) solution for pre-clinical and clinical cell and gene therapy research.
What is targeted sequencing?
Targeted sequencing is a technique that uses NGS to target specific genes, coding regions of the genome, or chromosomal segments for rapid identification and analysis of genetic mutations.
Why use targeted sequencing?
Targeted sequencing is useful for studying gene variants in selected genes or specific regions of the genome, since a large diversity of organisms is targeted rapidly and cost-effectively. Targeted sequencing is used to examine gene interactions in specific pathways and is faster and more cost effective than whole genome sequencing (WGS). This is because a smaller set of nucleotides are used, rather than broadly sequencing the entire genome.
Targeted sequencing Gene Panels
GENEWIZ from Azenta provides a variety of targeted resequencing panels, either pre-made or custom, that can cover any genes of interest or cancer-implicated genes.
Visit these pages to learn more about these services:
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Cancer Panels
Including commercially available hotspot cancer panels, as well as Azenta’s pan-cancer and OncoGxOne™ discovery cancer panels.
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TargetGxOne™ (Custom Targeted Sequencing)
Our custom panels can be designed to target any genes of your choosing.
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Whole Exome Sequencing
A cost-effective alternative to whole genome sequencing, which allows genetic analysis of genomic coding regions.
Targeted Sequencing Applications
Targeted Sequencing Features & Benefits
Workflow for Targeted Resequencing Panels
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Experimental Design
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Targeting and Library Preparation
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Sequencing
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Bioinformatics
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Sample Submission Guidelines
Azenta accepts the following sample types for targeted resequencing projects:
- Purified genomic DNA
- Frozen cell pellets (mammalian or bacterial)
- PCR amplicons
- Bacterial colonies
- Formalin-fixed, paraffin-embedded (FFPE) thin sections (slides)
- Select tissues
- Blood
- Swab
Technical Resources
Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
Webinar| Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response
Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response.
Webinar | Demystifying End-to-End DNA Sequencing Solutions
In this co-hosted webinar with Twist Bioscience, learn how researchers design custom panels to sequence deeply and efficiently into regions, genes, and organisms of interest, as well as gain a greater understanding on how targeted sequencing can be combined with the latest transcriptomics and proteomics tools to reveal even more insights.
NGS PLATFORMS
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.
