DNA Libraries
GENEWIZ DNA Libraries offer a wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for our partially (NNK) or completely randomized (NNN) nucleotide libraries.
DNA and Variant Library Applications
Protein Engineering: Build and identify proteins with novel or improved stability and functional characteristics from a library of mutants.
Enzyme Engineering: Modify an enzyme for enhanced activity for use in industrial biotechnology.
Directed Evolution: Build a library of variants with your desired mutations for applications in evolutionary biology.
Synthetic Biology: Build new metabolic pathways with different combinations of genetic elements for applications in metabolic engineering.
Antibody Development: Test and identify mutant sequences with improved antigen expression.
CRISPR/Gene Editing: Create knock-out/knock-in variants for high-throughput screening.
DNA Library Services
Combinatorial (Classical)
Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements.
Trimer-Controlled (Smart)
Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity.
Variant
Introduce singular/scanning site-directed mutagenesis in a user-defined 24 base pair region or truncate a sequence to characterize and optimize the desired protein features and function.
sgRNA
Accelerate CRISPR screening programs with single guide RNA (sgRNA) libraries containing knock-out/knock-in variants. Choose from pre-made or custom generated offerings.
Featured Application: Antibody Development
The success of an in-vitro antibody discovery and development program is contingent on the diversity of functional candidates as well as the ability to enhance favorable characteristics. Choose Azenta’s combinatorial or trimer-controlled libraries to expedite your antibody discovery and development program while keeping the cost low.
- Combinatorial DNA libraries A powerful tool for determining and optimizing biomolecule function from a parent sequence. By coupling these libraries with a screening technology such as phage display, it becomes possible for researchers to both develop and test an immense number of mutant variants in a short time. Depending on the research goals, budget, and timeline, different mutagenesis techniques can be used to create variants. The below table compares three industry standard approaches.
| Library | Stop Codons | Risk of Mutation | ‘AT’ Rich Bias | Assigned Amino Acids | Screening Speed | Screening Costs |
|
Random (NNN) |
3 | High | Possible | No | Slow | $$$ |
|
Partially Random (NNK/NNS) |
1 | High | Possible | No | Moderate | $$ |
|
Trimer-Controlled |
0 |
Low |
None | Yes | Fastest | $ |
- Trimer-controlled libraries: An effective tool that significantly reduces screening efforts compared to conventional mutagenesis methods, empower researchers with the most accuracy, precision, and control as compared to other combinatorial DNA library techniques because of the extra synthetic effort devoted to restricting codon building blocks. Download the case study below to learn why researchers at a large bio-pharmaceutical company chose Azenta combinatorial trimer-controlled synthesis of antibody libraries to obtain a highly accurate mutant library.
DNA Library Deliverables
| Library Type | Classical Combinatorial Libraries (NNN/NNK/NNS) and Trimer- Controlled (Smart libraries) |
Variant | |
|
Library Format |
Linear DNA pool | Cloned Pool | Individual Clones |
|
Diversity |
Up to 1012 variants | Up to 109 variants | Customer defined |
| Vector | N/A | Customer defined | Customer defined |
| Quality Control | Sanger sequencing of up to 96 individual transformants (default) Additional NGS-based diversity analysis (optional) |
Sanger sequencing of up to 96 individual transformants (default) Additional NGS-based diversity analysis (optional) |
100% sequence accuracy guaranteed.- Restriction digest of final plasmid |
| Deliverables | 1-2 µg of linear dsDNA Certificate of Analysis (COA) with quality measurements. |
5-10 µg of plasmid DNA Certificate of Analysis (COA) with quality measurements. Large scale DNA preparation options ranging from µg to mg. |
2-5 µg of plasmid DNA (standard) Certificate of Analysis (COA) with quality measurements. Large scale DNA preparation options ranging from µg to mg. |
If you have custom requests or require any pre-project consultation, please contact our expert project management team at PM@azenta.com
DNA Libraries Features & Benefits
HOW TO ORDER
*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.
