SNP Genotyping Analysis and Detection

SNP analysis and identification may help predict an individual’s response to certain drugs, susceptibility to environmental factors, and risk for developing diseases. Alternatively, SNPs can be used to track inheritance of disease genes within families and provide insight into SNP linkage.

Sanger sequencing-based SNP genotyping can be performed on a small subset of samples or as part of large-scale projects. Whether you are using genotyping as your primary approach for SNP analysis and detection, or to confirm next generation sequencing and microarray results, Azenta can help accelerate your project. Let the Sanger sequencing experts at Azenta support your SNP genotyping research objectives with speed, quality, and reliability.

Azenta vs. In-House

Mutation Analysis Turnaround Time Comparison: Azenta versus In-house

AZENTA TURNAROUND: 3 DAYS

IN-HOUSE TURNAROUND: 2-4 WEEKS

Deliverables

All SNP Genotyping projects will receive a raw data file (Figure 1.) along with a final report identifying SNPs compared to the provided reference sequence (Figure 2.). Custom reports are also available upon request.

Example: BRAF c.1799T>A (V600E) Mutation in Melanoma

Mutations in BRAF are largely linked to malignant melanomas. Mutations at BRAF V600 are common indicators for responsiveness to treatment for melanoma patients.

Figure 1. Chromatogram
Chromatogram showing the heterozygous mutation at nucleotide position 1799 (T to A mutation at position 16 below) which correlates to amino acid position 600 (V to E mutation).

Figure 2. Final Report

A SNP genotyping final report shows the reference SNP ID number, the allelic mutation, and the flanking sequence with the highlighted position of the identified SNP.