CLIA Variant Confirmation (PCR + Sanger)
PCR + Sanger sequencing allows you to detect or confirm mutations in genomic DNA. This technique is a reliable method for initial analysis or orthogonal confirmation of next generation sequencing, microarrays, or qPCR/dPCR results in clinical samples. Achieve clinical success in our GCP-compliant and CLIA-certified laboratory.Request Quote
Service Details
| Service Level* | Raw Data Files | QA Oversight | Variant Report | Lab Director Signature | Test Registration | Clinical Outcome Report |
| CLIA Environment | ||||||
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CLIA Workflow (With QA) |
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| CLIA (Full Service) |
Included | Available as an add-on | Not Available
*All service levels are performed by CLIA-trained personnel in a CLIA-certified lab with CLIA-qualified equipment.
Applications
SAMPLE TO ANSWER
1. PROJECT CONSULTATION & PRIMER DESIGN
Amplicon selection and primer design to targeted regions of interest in new or existing assays
2. ASSAY DEVELOPMENT
Optimization on project-specific samples with different validation options
3. PCR & PURIFICATION
PCR amplification of genomic DNA using ideal conditions
4. SEQUENCING & DATA ANALYSIS
Bi-directional DNA sequencing per amplicon with optional variant reporting
Deliverables
All customers receive raw data as .ab1, .seq, and .phd files. A Variant Report is available as an add-on for CLIA Environment and CLIA Workflow. An optional lab director signed quality score report is available with CLIA Workflow. CLIA (Full Service) includes both reports.
REQUEST YOUR CLIA VARIANT CONFIRMATION QUOTE
Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)
