ANTIBODY DISCOVERY SOLUTIONS
From Discovery to Pre-Clinical Development
INTRODUCTION
Antibody-based therapeutics have entered the center stage of drug discovery as a result of a major shift in focus from small molecules to a broader portfolio containing both protein and chemical therapeutic agents. The field is benefiting from both an increased understanding of the mechanistic basis of antibody-derived therapeutics and the development of sophisticated technologies to derive safe and targeted biotherapeutics.
ANTIBODY DISCOVERY WORKFLOW
GENEWIZ offers the latest sequencing technologies and customizable synthetic DNA solutions to expedite your discovery process while keeping the cost low.
Check out GENEWIZ antibody solutions below to help you with each step of the discovery process.
Step 1: Library Generation
Synthetic DNA Solutions
Synthetic DNA Libraries
provide highly diverse synthetic variant and combinatorial libraries with unprecedented flexibility to customize amino acid diversity to optimize your antibody discovery process.
Next Generation Sequencing
Diversity Assessment
via Amplicon Sequencing provides a comprehensive diversity analysis of your synthetic antibody DNA libraries.
Step 2: Lead Identification
Molecular Genetics
PCR + Sanger Assay
offers a full-service solution to facilitate the screening and confirmation of heavy chain/ light chain sequences.
Sanger Sequencing
Direct Colony Sequencing
enables rapid phage analysis of isolated antibody clones after a bio-panning affinity selection.
Next Generation Sequencing
Amplicon Sequencing
offers identification of highly diverse leads after bio-panning enrichment.
Immuno-Profiling
provides comprehensive analysis of in vivo repertoires after immunization.
Step 3: Lead Optimization
Synthetic DNA Solutions
Antibody DNA Synthesis
offers fully codon-optimized synthetic antibody constructs for recombinant antibody expression starting at just 6 business days.
Sanger Sequencing
Colony Sequencing + Plasmid DNA Prep
offers a full-service solution to directly sequence bacterial clones followed by large-scale DNA preparation for recombinant antibody expression.
CRISPR Solutions
Genome Editing and Engineering
offers a wide range of synthetic DNA solutions and sequencing-based solutions for creating transgenic cell lines.
Long Single-Stranded DNA Synthesis
enables CRISPR-mediated gene knock-in to integrate recombinant V(D)J region to generate transgenic models for antibody production.
Step 4: Pre-Clinical Development
GLP-Compliant Services
GLP-Compliant Sequencing
of antibody-producing cell lines for FDA level submissions and confirmation of manufactured cell banks throughout the clinical pipeline.
GLP-Compliant Plasmid Prep Services
offer a range of DNA preparation solutions to accelerate research and pre-clinical development for antibody drug discovery.
Next Generation Sequencing
Whole Genome Sequencing
provides a comprehensive validation of antibody-expressing cell lines on multiple sequencing platforms.
THE GENEWIZ DIFFERENCE
Stringent Quality Control
Optimized workflows and technical expertise produce consistent results with best-in-class quality. GLP, CLIA, and CAP services are available.
Industry-leading Turnaround Time
DNA sequencing and synthesis delivered when you need it with expedited options for many services.
Unparalleled Technical Support
Ph.D.-level consultation provides guidance before, during, and after the project.
Partner of Choice
Over 600 pharmaceutical and biotechnology companies have trusted us with their projects.
CONTACT GENEWIZ
Ph.D.-level experts are available to discuss your project. Call us today at 877-436-3949.
Next Generation Sequencing • Ext. 1 • ngs@genewiz.com
Sanger Sequencing • Ext. 2 • dnaseq@genewiz.com
Synthetic DNA Solutions • Ext. 3 • pm@genewiz.com
Molecular Genetics • Ext. 3350 • molgen@genewiz.com
GLP-Compliant Services • Ext. 3350 • regulatory@genewiz.com