Sanger Tips & Tricks

Learn from the Sanger experts how you can get the most of your DNA Sequencing

•  Download all Volumes
•  Download Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template
•  Download Volume 4: PCR Visualization by Gel Electrophoresis
•  Download Volume 3: Salvaging Nonspecific PCR Products
•  Download Volume 2: PCR Clean-up of Single-band PCR
•  Download Volume 1: Producing Robust, Single-band PCR Product

An Efficient and High-Fidelity Approach to AAV Plasmid Preparation (Tech Note)

Preserving the integrity of inverted terminal repeat (ITR) sequences in adeno-associated virus (AAV) plasmids is important to produce recombinant AAV (rAAV) vectors. Azenta’s proprietary AAV plasmid preparation service delivers superior quality for midi to giga-scale AAV vectors, while maintaining the integrity of ITR regions. Our new process includes pre- and post-preparation quality controlled (QC) steps that leverage Azenta’s AAV-ITR Sanger sequencing capabilities for full sequence verification and ensures delivery of intact ITR clones for your research.

AAV ITR Sequencing Tech Note

Azenta has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.

Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal (Tech Note)

This tech note describes how Azenta scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data on the 10x Genomics® Chromium™ platform.

Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq (Case Study)

Using only ~50 tumor cells as input, Azenta generated high quality transcriptomic data that enabled researchers to publish in a top journal.

Single-Cell RNA-Seq Analysis Identifies Rare Drug – Resistant Cancer Stem Cells (Case Study)

Discover the value of scRNA-seq in identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers in this Azenta-exclusive study.

Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT^TM (Case Study)

Discover the powerful capability of genoTYPER-NEXT in this illuminating case study that discusses this new technology and how it’s helping researchers analyze NGS DATA.

Trimer-Controlled Synthetic Libraries (Case Study)

Download this case study now to learn why researchers at a large bio-pharmaceutical company chose Azenta’s Trimer-Controlled Library solution to obtain a highly accurate mutant library.

Competent Cell Selection Guide

This guide will help you choose protocols for making competent cells and appropriate strains for your experiment.

Multiplexing microbes on the PacBio Sequel: Feasibility, benefits, and recent developments (Tech Note)

This study proves the feasibility of multiplexing and highlights the key advantages of the PacBio Sequel platform as compared to its predecessor, the RSII.

Detection of Previously Unidentified Structural Variants Involved in Glioblastoma Using PacBio Sequel^TM Sequencing

This case study looks at how Azenta utilized the PacBio® Sequel™ long-read technology to help a customer improve detection of structural variants within glioblastoma samples.