Metagenomic Sequencing

Metagenomic sequencing uses next generation sequencing (NGS) to rapidly analyze microbial community diversity and understand the role of constituent species. Our metagenomic sequencing solutions range from targeted assays, such as 16S for prokaryotes and ITS2 for fungi, to shotgun sequencing and metagenomic analysis. Explore below which service best fits your needs, or check out our webinar, “Uncovering Hidden Microbial Diversity with NGS Technology,” to learn more about our capabilities.

What is metagenomic sequencing?

Metagenomic sequencing is the collective analysis of all genomic material present in a sample (containing mixed microorganism communities), thus eliminating the need for isolating and cultivating individual organisms.

What is metagenomics vs. genomics?

Both metagenomics and genomics are fields of study within molecular biology, but the difference is in scope. While genomics is focused on the genome of a single organism, metagenomics encompasses all genomes of various microorganisms within a single given sample, providing a broad overview of an ecosystem’s composition.

Metagenome Sequencing Service Options

	16S-EZ	Fungal Identification	Whole Genome Metagenomics
Microbes Targeted	Bacteria and Archaea	Fungi	All
Taxon Level	Genus/Species	Genus/Species	Sub-species
Genomic Loci Sequenced	16S rRNA gene (V3 and V4 regions)	Internal Transcribed Spacer 2 (ITS2)	Whole Genomes
Purpose of Assay	Taxon Identification		Taxon identification Variant discovery
Benefits	Enhanced sensitivity outperforms V4-only assays	High variation in ITS2 region allows differentiation between closely related species	Most comprehensive assay – identify over 53,000 microbes Phylogenetic analysis Ideal for organisms with poorly annotated 16S genes
Available Reports	Rank-abundance curve Taxonomic classification Rarefaction curve Principal coordinate analysis Hierarchical clustering		Taxonomic classification of reads across all ranks Microbial abundance estimation across all ranks Cross-sample comparisons across all ranks Dynamic, visual exploration of microbial taxonomy Interactive, searchable data tables

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Technical Resources

Webinar | Uncovering Hidden Microbial Diversity with NGS Technology

This webinar outlines the role and importance of microorganisms in our everyday world, how to classify and identify them, and the next generation sequencing techniques that work best for these tasks. We touch on the wide array of areas that microbial genomics can be applied to and the NGS case studies that have helped scientists thrive in various research areas.

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Tech Note: 16S MetaVx™ Environmental Detects Greater Prokaryotic Diversity Than Traditional 16S Assay

We compared GENEWIZ’s 16S MetaVx Environmental service against a traditional V4-only assay. 16S MetaVx Environmental consistently detected a greater number of bacteria and archaea, demonstrating its superior sensitivity and specificity.

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Please inquire for more details. (ngs@genewiz.com)

Webinar | Understanding biological mechanisms to better predict the evolution of antibiotic resistance

The rapid and wide-spread evolution of antibiotic resistance is threatening global health. In this webinar, presented by Dr. Mato Lagator, Wellcome Trust, Royal Society Sir Henry Dale Fellow, University of Manchester, you will learn about different approaches to study evolutionary processes that underpin the emergence of resistance. Special focus is given on how these methods can be utilized to improve drug development and longevity.

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Deliverables

All customers receive their raw data as FASTQ files. MetaVx2.0™ provides an enhanced report through analysis with the QIIME software suite. This optional, enhanced report includes figures such as rank-abundance curves, taxonomic classification, rarefaction curves, principal coordinate analysis and hierarchical clustering analysis. An interactive taxonomic viewer detailing different taxa and their relative abundance in each sample is also available by request.