Modified Oligos
Modified Oligo services are designed to equip researchers with customized oligos featuring a wide variety of modifications. Choose your synthetic scale, HPLC or PAGE purification options, and delivery format without paying premium prices. Spend more time innovating and less time worrying about your budget, with custom oligo solutions you can depend on.
Modified Oligo Applications
Oligo Modification Services
DNA Oligo Modifications
With your choice of over 200 chemical modifications, our standard oligo synthesis services provide the high-quality results you expect from Azenta with the breadth of oligo synthesis expertise you need for your smaller DNA synthesis experiments.
Custom Oligo Projects
Azenta offers nearly any oligo solution. Mixed bases, NGS adapter primers, oligo pools, DEL libraries, special delivery scales/formats, and more are accessible through our custom online ordering form to meet your exact project requirements.
RNA Oligos
Synthetically designed RNA is used in research applications such as gene function analysis and therapeutic development. Azenta’s expertise in oligonucleotide synthesis ensures your RNA, modified RNA, and RNA/DNA chimeras are
delivered quickly and in high-quality.
Featured Application: Gene Editing
RNA oligos are used to control transcription, translation, and a host of other cellular activities. Because of their central role in molecular biology, RNA can function as important therapeutics and probes for genetic function. Additionally, they are critical components of gene editing programs to provide templates for CRISPR/Cas9 endonuclease activity. Complete single guide RNA (sgRNA) constructs from Azenta enable gene knock-out/knock-in experiments without the need for an extra annealing step between crRNA and tracrRNA subunits.
snoRNA
Facilitates chemical modification of RNAs
miRNA
Regulates gene expression
siRNA
Silences gene expression
snRNA
Functions in various nuclear processes (e.g. splicing)
sgRNA
Edit genes with CRISPR/Cas9 systems
Discover the library of RNA structures and modifications available from Azenta.
Popular Oligo Modifications
Below are just a few examples of common DNA oligo synthesis services and modifications ordered by Azenta customers. Contact our Ph.D. technical support team for a comprehensive list of over 200 modifications, more information, or guidance on custom synthetic needs for any research project in your lab.
Attachment modifications
Attachment modifications offer benefits to many fundamental research and molecular diagnostic applications. Biotinylation and digoxigenination are functional in immunoassays (protein detection; ELISA), hybridization (Southern blotting; Northern blotting; in situ), cellular staining, and pull-down isolation experiments. Aminolinkers and thiols both provide functional handles for further attachments either internally or at 5’ or 3’ positions.
Quencher Modification
Quenchers are used in conjunction with a fluorophore to investigate DNA and/or RNA interactions. These systems are typically part of FRET or reporter-quencher experiments.
Fluorescent Dye Modification
Dyes are generally used to localize DNA and/or RNA sequences of interest in the cell. Choose from a variety of fluorescent dyes with a wide spectrum of absorption, emission, and color depending on the needs of your experiment and detection technique.
Modified Bases
Modified nucleosides such as deoxyinosine (dI) provide degeneracy in DNA oligos with limited sequence information, while deoxyuridine (dU) improves double stranded stability (and associated melting point) as a replacement for deoxythymidine. Phosphorothioate oligos are particularly useful in antisense experiments to inhibit nuclease activity.
5′ and 3′ Phosphorylation
5′ phosphorylation can be used for linkers, cloning and gene construction, and ligase- catalyzed ligation reactions. Phosphorylation is also used to prevent DNA chain extension catalyzed by DNA polymerase in related experiments that are resistant to exonuclease digestion.
Features and Benefits
OLIGO Purification Options
DNA oligonucleotides are provided in either a tube or plate format, fully lyophilized after purification. Below are recommended purification techniques based on DNA oligo length and required purity.
| Purification | Applicable Length | Advantages |
| Desalted Purification | 5-90 nt | Fast turnaround time and favorable price |
| PAGE Purification | 21-150 nt | |
| High purity >90%; suitable for long oligos | ||
| HPLC Purification | 5-90 nt | High purity >95%; suitable for modified oligos |
