Oligos Retained for Sanger
GENEWIZ Oligos Retained for Sanger service eliminates the need to coordinate between vendors before sending your samples for Sanger sequencing. We synthesize, dilute, organize, and store your primers for up to a year for use with future Sanger sequencing orders. This service is designed for the bench scientist with simplicity and convenience in mind.
How are oligos used in Sanger sequencing?
Oligos are used in Sanger sequencing as primers to initiate DNA synthesis and as terminal nucleotides to terminate further DNA synthesis.
Features & Benefits
Turnaround Time
Order Placed |
US (Non-Boston Area) |
|
| Before 3pm ET | Ready for sequencing the next business day* | |
Order Placed |
Greater Boston Area |
|
| Before Noon | Ready same-day for overnight sequencing* | |
| Noon – 8pm | Ready for sequencing the next business day* | |
*Larger orders or sequencing oligos longer than 30 bases may require additional turnaround time. Oligos Retained for Sanger will be transferred internally for Azenta sequencing projects. Please contact DNASeq@azenta.com for more information.
Technical Resources
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
