Tools for Viewing Sanger Sequencing Data
Sequence / Chromatogram Viewing Software
A number of free software programs are available for viewing trace or chromatogram files. Click on the appropriate icon(s) to go to the respective Web page. You will find information about downloading, installing and using the software. Please make your choice based on your computer platform and operating system.
Sanger Data Analysis
| Free/Paid | Trace File Viewer | Multiple Sequence Alignment & Contig Assembly | Windows | Mac | Linux | In-browser | |
| 4Peaks | Free | – | – | – | – | ||
| Sequence Scanner | Free | – | – | – | – | ||
| Chromas Lite | Free | – | – | – | – | ||
| SnapGene Viewer | Free | – | – | ||||
| GeneStudio Pro | Free | – | – | – | |||
| UGENE | Free | – | |||||
| Chromaseq | Free | – | |||||
| BioLign (Note: See bottom half of page for description and download link) |
Free | – | – | – | – | ||
| CAP3 (Note: Only for contig assembly of sequences in FASTA format) |
Free | – | – | – | – | ||
| Geneious | Paid | – | |||||
| SeqMan Pro | Paid | – | – | ||||
| Sequencher | Paid | – | – | ||||
| Vector NTI Express | Paid | – | – | ||||
| Chromas Pro | Paid | – | – | – | |||
| CodonCode Aligner | Paid | – | – | ||||
| DNA Baser | Paid | – | – | – |
Free Molecular Cloning Software
| Windows | Mac | Linux | |
| SnapGene Viewer | |||
| Serial Cloner | |||
| UGENE | |||
| ApE (A Plasmid Editor) | – |
Decompression Software
WinZipFree Online Primer Design Software
Primer3NCBI Primer-BLAST
Free Online Oligo Analysis Tools
Oligo Calc