Gene Fragments
Gene Fragments are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that Azenta Life Sciences, formerly GENEWIZ has developed for standard gene synthesis products. We provide Gene Fragments in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.
How are gene fragments made?
Construction begins with the base-by-base synthesis of oligonucleotides (oligos), followed by assembly into double-stranded DNA (dsDNA) fragments. These custom DNA fragments can be used directly, cloned into vectors, or assembled into larger constructs to serve a variety of research uses.
Gene Fragment Synthesis Features and Benefits
Cloning accuracy: More than 85% of the recombinant colonies from cloning Gene Fragments will contain your desired insert
Fast delivery time: Fast turnaround time starting in just a few days to meet your research deadlines
Affordable: Very cost-effective to meet your strict budget constraints
Quality control: Stringent quality control process to ensure high sequence fidelity
Gene Fragment Turnaround Time
| Sequence Length* | Quantity | Completion Time** | Predicted Cloning Accuracy |
| 100-500 bp | 500 ng | 2-4 business days | >85% |
| 501-750 bp | 3-5 business days | ||
| 751-1,000 bp | 1,000 ng | ||
| 1,001-1,500 bp | 4-6 business days | ||
| 1,501-2,250 bp | 6-8 business days | ||
| 2,251-3,000 bp |
*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.
Gene Block Quality Control
- Size verification by gel electrophoresis
- Sequence confirmation via Sanger sequencing
Gene Fragment Synthesis Applications
- Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
- gRNA expression cassettes for CRISPR/Cas9-based gene editing
- Donor constructs for gene editing experiments
- Template for in vitro transcription
Gene Fragment Assembly Deliverables
Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.
HOW TO ORDER
*Samples must arrive at the Azenta New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.
