PCR + Sanger (R&D, GLP)

Sample Submission Guidelines

View our Sample Submission Best Practices Checklist.

qPCR/RT-qPCR

Sample Type	R&D	GLP
Fresh frozen tissue	At least 2 mg (10 mg recommended)	By inquiry
Cell pellets	At least 10⁴ cells (10⁶ cells recommended)	By inquiry
DNA	At least 2 µg (5 µg recommended)	By inquiry
RNA or cDNA	At least 2 µg; recommended quality for Total RNA samples is RIN≥6 (5 µg recommended)	By inquiry

dPCR

Sample Type	R&D	GLP
Fresh frozen tissue	At least 2 mg (10 mg recommended)	At least 2 mg (10 mg recommended)
Cell pellets	At least 10⁴ cells (10⁶ cells recommended)	At least 10⁴ cells (10⁶ cells recommended)
Whole blood	DNA extraction: 2 tubes of 200ul (500ul/tube recommended) RNA extraction: 2 tubes of 2.5mL (whole blood, PAXgene Blood RNA Tube)	DNA extraction: 2 tubes of 200ul (500ul/tube recommended) RNA extraction: 2 tubes of 2.5mL (whole blood, PAXgene Blood RNA Tube)
FFPE samples	2 slices per sample, 5-10 µm per slice/curl (≥4 FFPE slides recommended)	2 slices per sample, 5-10 µm per slice/curl (≥4 FFPE slides recommended)
Viral particles	Cell-free viral particles: 1 x 10⁷ to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred Frozen allantoic fluid: at least 1 ml	Cell-free viral particles: 1 x 10⁷ to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred Frozen allantoic fluid: at least 1 ml
Swab	2 tubes/sample, 1 swab/tube	2 tubes/sample, 1 swab/tube
DNA	DNA concentration should be between 2-50 ng/µl (100ng recommended per target); A260/A280 and A260/A230 ratios should be at least 1.8 to ensure high-quality DNA	DNA concentration should be between 2-50 ng/µl (100ng recommended per target); A260/A280 and A260/A230 ratios should be at least 1.8 to ensure high-quality DNA
RNA or cDNA	At least 2 µg; recommended quality for total RNA samples is RIN≥6 (5 µg recommended)	At least 2 µg; recommended quality for total RNA samples is RIN≥6 (5 µg recommended)

Nucleic Acid Extraction

Sample Type	R&D	GLP
Frozen mammalian cell pellet samples	At least 1 X 10⁶ to 1 x 10⁷ cells per pellet*	At least 5 x 10⁶ cells per pellet (2 pellets preferred), recommended 1 x 10⁷ cells per pellet*
Frozen mammalian tissue	At least 25 mg*	At least 25 mg*
FFPE Tissue (slides or curls)	1-2 slices per sample, 5-10 µm per slice/curl	Not available
Blood samples	200 µl blood	200 µl blood
Buccal swabs	At least 1 per sample	Not available
Saliva	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

SNP/Mutation Analysis

Sample Type	R&D	GLP
Genomic DNA samples	≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer*	At least 200 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples	At least 1 x 10⁶ to 1 x 10⁷ cells per pellet*	At least 5 x 10⁶ cells per pellet (2 pellets preferred), recommended 1 x 10⁷ cells per pellet*
Frozen mammalian tissue	At least 25 mg*	At least 25 mg*
FFPE Tissue (slides or curls)	1-2 slices per sample, 5-10 µm per slice/curl	Work can be performed on already extracted material from this sample type
Blood samples	200 µl blood	200 µl blood
Yeast Colony	Agar plate with single and well isolated colonies	Agar plate with single and well isolated colonies
Buccal swabs	At least 1 per sample	Not available
Saliva	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

cDNA Verification

Sample Type	R&D	GLP
RNA	At least 5 µg*	At least 5 µg*
cDNA	At least 200 ng per sample, conc 20 ng/µl*	At least 200 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples	At least 1 x 10⁶ to 1 x 10⁷ cells per pellet*	At least 5 x 10⁶ cells per pellet (2 pellets preferred), recommended 1 x 10⁷ cells per pellet*
Frozen mammalian tissue	At least 25 mg*	At least 25 mg*
FFPE Tissue (slides or curls)	1-2 slices per sample, 5-10 µm per slice/curl	Work can be performed on already extracted material from this sample type
Blood samples	200 µl blood	200 µl blood
Viral particles	If cell free viral particles: 1 x 10⁷ to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred* If frozen allantoic fluid: at least 1 ml*	If cell free viral particles: 1 x 107 to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred* If frozen allantoic fluid: at least 1 ml*

gDNA Verification

Sample Type	R&D	GLP
Genomic DNA samples	≥60 ng per amplicon per sample, conc 20 ng/µl, in water, elution buffer, or low TE buffer*	At least 120 ng per sample, conc 20 ng/µl*
Frozen mammalian cell pellet samples	At least 1 x 10⁶ to 1 x 10⁷ cells per pellet*	At least 5 x 10⁶ cells per pellet (2 pellets preferred), recommended 1 x 10⁷ cells per pellet*
Frozen mammalian tissue	At least 25 mg*	At least 25 mg*
FFPE Tissue (slides or curls)	1-2 slices per sample, 5-10 µm per slice/curl	Work can be performed on already extracted material from this sample type
Blood samples	200 µl blood	200 µl blood
Virus	If cell free viral particles: 1 x 10⁷ to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred* If frozen allantoic fluid: at least 1 ml*	If cell free viral particles: 1 x 10⁷ to 1 x 10¹²/particles per ml, submit 1 ml, 2 vials preferred* If frozen allantoic fluid: at least 1 ml*
Buccal swabs	At least 1 per sample	Not available
Saliva	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)	Preserved saliva, 1 collection tube per sample (Follow manufacturer’s guidelines for collection amount)

Fragment Analysis

Sample Type	R&D	GLP
Labeled PCR products	10-20 µl equivalent of PCR product	Not available

16S rRNA and Fungal ITS Sequencing

Sample Type	R&D	GLP
Bacterial colony on agar	Agar plate with single and well isolated colonies	Not available
gDNA	At least 60 ng per sample, conc 20 ng/µl, homogeneous samples only for this service	Not available
Glycerol stocks	200 µl of frozen stock on dry ice, homogeneous samples that can be pelleted when thawed (≥10⁶cells)	Not available
Agar stabs	Agar stab with clonal sample	Not available
Cell Pellets	≥10⁶cells, dry or resuspended in buffer	Not available
Fungal colony on agar	Agar plate with single and well isolated colonies	Not available

Plasmid Sequencing

Sample Type	R&D	GLP
Plasmid DNA	Primer Walking	At least 5 µg* If transforming- At least 500 ng
Glycerol stock	Primer Walking	>200 µl of frozen stock on dry ice, 2 stocks preferred*
Bacterial colony on agar	Primer Walking	Agar plate with single and well isolated colonies

Yeast Colony Sequencing

Sample Type	R&D	GLP
Yeast Colonies	Agar plate with single and well isolated colonies	Agar plate with single and well isolated colonies

Large-Scale DNA Preparation

Sample Type	R&D	GLP
Plasmid DNA	Preps	At least 5 µg If transforming – At least 500 ng
Glycerol stock	Preps	200 µl of frozen stock on dry ice, 2 stocks preferred*
Bacterial colony on agar	Preps	Agar plate with single and well isolated colonies

*Requires Dry-Ice Shipment

SHIPMENT INSTRUCTIONS FOR ALL R&D-LEVEL SERVICES

Recommended Packaging and Shipping Guidelines:

Please secure all samples for shipment as appropriate:

≤ 36 samples: Please submit samples in 1.5 ml snap-lock microcentrifuge tubes or 8-strip tubes with securely fastened lids. Parafilm can be used to minimize risk to samples during shipment.
>36 samples: Please submit samples arrayed on a 96-well plate, sealed with an appropriate adhesive plate seal and centrifuged to collect samples in the wells.
Agar plates: Please ship agar plates in a sturdy container with the agar-side up to prevent condensation build-up. Secure agar plate lids with tape, rubber bands, or parafilm to minimize risk to samples during shipment.
Glycerol stocks, RNA, and samples for extraction should be shipped using dry ice. Please consider the estimated delivery date of your shipment when determining the amount of dry ice to use.

Note: GENEWIZ accepts purified genomic DNA or can extract genomic DNA from various sample types, including biosafety level 1 (BSL1) and 2 (BSL2) supplied sources. We do not accept samples that require a higher level of processing than BSL 2.

Direct Shipping (FedEx, UPS or World Carrier):

If a Drop Box is not available, or if your starting material requires dry ice, please use an overnight delivery service such as FedEx, UPS, or DHL. A print-out of the Excel order page must be included with the material.

All direct shipments should be sent to:
GENEWIZ
Attn: Molecular Genetics
115 Corporate Blvd
South Plainfield, NJ 07080

GENEWIZ Drop Box (if available):
Starting material that is stable at room temperature may be submitted via a GENEWIZ Drop Box (when available). A print out of the Excel order page must be included with the material. Contact dnaseq@genewiz.com to request a Drop Box pick-up for your location or if you have any Drop Box questions.

SHIPMENT INSTRUCTIONS FOR ALL GLP-COMPLIANT SERVICES

All shipments via FedEx, UPS or World Courier should be sent to:

Dry ice, ship to:
Attn: GLP Services
111 Corporate Boulevard
South Plainfield, NJ 07080
USA

Please include a copy of the Sample Registration Form.