CRISPR gRNA Synthesis (CRISPR Construct Synthesis)
CRISPR gRNA Synthesis service can synthesize the CRISPR sequence, clone it into your vector of choice, and perform sequence verification. CRISPR gene editing makes highly flexible, highly specific, and affordable genome engineering possible. Enabling almost any genomic target to be modified in any organism that can express exogenous DNA, CRISPR/Cas9 and other CRISPR-associated systems continue to revolutionize the field of genome engineering. This service is labeled as CRISPR Construct Synthesis in our CLIMS4 ordering system.
What is gRNA?
Guide RNA (gRNA) is an RNA sequence that serves to guide the editing process – directing the CRISPR associated nuclease (Cas nuclease) over to the target DNA region of interest.
What is synthetic sgRNA?
Synthetic sgRNA, or single guide RNA, is a synthetically generated single RNA molecule that contains the nucleotide sequence complementary to the target DNA (also known as CRISPR RNA or crRNA for short) and a tracrRNA that enables the Cas nuclease to bind.
What is the difference between sgRNA and gRNA?
The difference between sgRNA (single guide RNA) and gRNA (guide RNA) is that sgRNA contains both crRNA and tracrRNA fused into one RNA molecule. Meanwhile, gRNA (guide RNA) is a general term for all guide RNAs used with CRISPR. Synthetic sgRNA is better optimized for genome engineering compared to other guide RNAs, as it allows for fast, reliable, and reproducible editing results.
gRNA Synthesis and Cloning
Azenta offers very competitive pricing to synthesize, clone and sequence verify your gRNA expression cassettes into your preferred vector regardless of vector size or complexity. Our strong expertise and experience in highly reliable synthetic construct generation services coupled with our CRISPR offering makes us a valuable partner to researchers around the globe. Simply provide your pre-design sequence to Azenta for any of the CRISPR-related products below and receive a quote within one business day:
gRNA Synthesis and Cloning
Azenta will synthesize and clone your gRNA sequences as cDNA into your preferred vector regardless of vector size and complexity, using the same industry-leading proprietary technology that Azenta has developed for standard gene synthesis products.
Homology Directed Repair (HDR) Donor Construct and Custom Vector Synthesis
Need a custom donor or vector construct synthesized? Azenta has years of experience working with complex and unstable vectors. We can provide custom constructs for your HR-mediated repair template as well as custom CRISPR vector generation and modifications. All custom vectors can be stored in-house for your future orders for up to 2 years at no additional cost.
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Standard Deliverables:
– 2-5 µg of lyophilized plasmid containing your sequence verified synthetic construct
– Certificate of Analysis (COA) including restriction digest
– Sequence trace data with alignment
– Sequence files of synthetic gene alone and in vector
Synthetic Libraries
Looking to create a gRNA library to screen knock-outs/knock-ins of a variety of genes in one experiment? Azenta custom synthetic library service can create a custom gRNA library for your high-throughput CRISPR screening needs.
TYPICAL CLONED GENE SYNTHESIS WORKFLOW
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Oligonucleotide Synthesis
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Gene Assembly
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Cloning
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Quality Control
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Synthetic Gene Shipment
5
HOW TO ORDER
*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.
