Free Universal Primers
Azenta offers a variety of free universal primers for sequencing. These free universal primers are being updated to reflect the needs of our customers. Users in our new CLIMS Online Ordering and Data Management System have access to the Updated Azenta Universal Primer list (see below). If you are unsure which CLIMS system you are using, please contact our Technical Support team at 877, ext. 2.
Primer not listed? Learn more about Azenta Oligos.
| Updated Azenta Universal Primers | |||
|---|---|---|---|
| Name | Length(nt) | Sequences(5′-3′) | |
|
T7 |
20 |
TAATACGACTCACTATAGGG |
|
|
T7 Term |
19 |
GCTAGTTATTGCTCAGCGG |
|
|
T3 |
20 |
ATTAACCCTCACTAAAGGGA |
|
|
SP6 |
19 |
GATTTAGGTGACACTATAG |
|
|
U6 |
20 |
GACTATCATATGCTTACCGT |
|
|
BGHR |
18 |
TAGAAGGCACAGTCGAGG |
|
|
M13F |
16 |
GTAAAACGACGGCCAG |
|
|
M13R |
17 |
CAGGAAACAGCTATGAC |
|
|
M13-40FOR |
17 |
GTTTTCCCAGTCACGAC |
|
|
M13-48REV |
20 |
CGGATAACAATTTCACACAG |
|
|
CMV-Forward |
21 |
CGCAAATGGGCGGTAGGCGTG |
|
|
5GEX |
23 |
GGGCTGGCAAGCCACGTTTGGTG |
|
|
3GEX |
23 |
CCGGGAGCTGCATGTGTCAGAGG |
|
|
pFastBacF |
20 |
GGATTATTCATACCGTCCCA |
|
|
pFastBacR |
20 |
CAAATGTGGTATGGCTGATT |
|
|
pBAD Forward |
20 |
ATGCCATAGCATTTTTATCC |
|
|
pBAD Reverse |
18 |
GATTTAATCTGTATCAGG |
|
|
EGFP-C-For |
22 |
CATGGTCCTGCTGGAGTTCGTG |
|
|
EGFP-C-REV |
17 |
GTTCAGGGGGAGGTGTG |
|
|
EGFP-N |
21 |
CGTCGCCGTCCAGCTCGACCA |
|
|
SV40pA-R |
20 |
GAAATTTGTGATGCTATTGC |
|
|
ATTB1 |
20 |
GTTTGTACAAAAAAGCAGGC |
|
|
ATTB2 |
24 |
CCACTTTGTACAAGAAAGCTGGGT |
|
|
ATTL1 |
24 |
CGCGTTAACGCTAGCATGGATCTC |
|
|
ATTL2 |
20 |
CATCAGAGATTTTGAGACAC |
|
|
ITS1 |
19 |
TCCGTAGGTGAACCTGCGG |
|
|
ITS4 |
20 |
TCCTCCGCTTATTGATATGC |
|
|
PJET1-2F |
23 |
CGACTCACTATAGGGAGAGCGGC |
|
|
PJET1-2R |
24 |
AAGAACATCGATTTTCCATGGCAG |
|