Purified Templates
GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences provides reliable, award-winning Sanger DNA Sequencing for purified templates through our Pre-Mixed, Pre-Defined, Custom and Difficult Template sequencing service options. Sanger sequencing from purified templates is our most popular template type. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, GLP, biotechnology, and government institutions.
SANGER SEQUENCING OF PURIFIED TEMPLATES
Azenta offers three service levels for Sanger sequencing of purified plasmid and PCR templates: Pre-mixed, Pre-defined, and Custom.
To determine the service that is best for your samples, please see the descriptions for each service below.
If you need help choosing your optimal Sanger service level, please contact us:
Email | Phone (1-877-436-3949, Ext. 2) | Live Chat
Pre-Mixed
For sequencing plasmids or purified PCR products, this service is the most economical choice.
You provide your template and primer mixed in the same tube according to our Sample Submission Guidelines.
Pre-Defined
By choosing our Pre-Defined Service, you submit your template and primer in separate tubes, and a Azenta scientist will mix them when your sample is received. An additional charge applies in most cases.
For sequencing plasmids or purified PCR products, submit your template and primer in separate tubes according to our Sample Submission Guidelines.
Note: An Azenta Universal Primer can be used in this service at no charge.
Custom
This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, an Azenta scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.
Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.
Submit template and primer separately according to our Sample Submission Guidelines.
Note: An Azenta Universal Primer can be used in this service at no charge.
Difficult Template Sequencing
Revolutionize your sequencing experience with our Difficult Template sequencing service, specifically designed for challenging templates featuring GC-rich regions or intricate secondary structures like hairpins. With a success rate surpassing standard workflows by over double, researchers can now save at least a day in their process. Versatile and efficient, this protocol accommodates various DNA types without the need for additional templates, offering unparalleled convenience and reliability.
Learn more about our proprietary Sanger sequencing service – Difficult Template Sequencing
Features & BENEFITS
High Quality
DNA sequencing read lengths ~1000 bases and at least 500 bases Phred20. A typical read will provide 800 bases Phred 20. Quality Score and Contiguous Read Length (CRL) provided in each readConvenient
Contact us to locate a drop box or pick-up service near you for free shippingFree Vector Primers
Wide selection of Azenta Universal Primers are available for most standard vectorsTechnical Support
Available at no charge to discuss your project, troubleshoot, and answer questions 8 AM – 8 PM Eastern Time, Monday – Friday; results are posted on Saturdays for weekend workersOnline System
24/7 web-based online ordering and data retrieval, with bar-coded order trackingTechnology
Analysis using ABI 3730xl DNA Analyzers for capillary electrophoresis and fluorescent dye terminator detectionHOW TO ORDER
*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.
