Next Generation Sequencing: Amplicon-EZ
Amplicon sequencing made easy!
- Easy on your budget – as low as $58 per sample
- Easy to order – checkout online in minutes
- Easy to submit samples – use a Azenta Life Sciences, formerly GENEWIZ drop box
- Easy to analyze data – interactive reports with intuitive design
- Easy on your deadline – results in 8 business days*
GENEWIZ Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast.
Amplicon Sequencing in 8 Days*
1. Sample submission
- No minimum sample number
- Convenient sample submission via dropboxes
2. Library Preparation
- 150-500 bp products accepted
- Submit with or without lllumina® partial adapters
3. Sequencing
- -50,000 reads per sample
- Full sequence coverage with 2x2S0 bp sequencing
4. Data Analysis
- Interactive variant report
- Unique sequence identification and abundance analysis
*Samples are processed at our New Jersey, USA facility in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).
Applications: CRISPR SCREENING & MORE
CRISPR Screening with Amplicon-EZ
1. Edit cells with CRISPR
2. Amplify target region from edited cell populations
3. Use Amplicon-EZ to expedite sequencing of PCR products
4. Analyze results to find wells with desired mutations**
CRISPR Validation with Amplicon-EZ
1. Isolate clones from postitive wells (see CRISPR screening)
2. Amplify target region from clones
3. Use Amplicon-EZ to expedite sequencing of PCR products
4. Validate edits and find homozygous mutants**
**View our interactive report (login required)
