Long-Read Amplicon Sequencing
GENEWIZ Long Read Amplicon Sequencing provides ultra-deep, contiguous amplicon sequencing for sequences up to 10,000 bp. A single PacBio® read can discover a wide range of variants, from SNPs and small indels to large insertions and deletions. Furthermore, circular consensus sequences can be generated to obtain up to 99.999% single-molecule base calling accuracy.
Single Molecule, Real-Time (SMRT®) long-read sequencing offers complete, uniform, nonbiased coverage spanning long amplicons. Unlike short-read technologies, SMRT sequencing does not require the amplicon to be fragmented, and allows for phasing of variants across the entire amplicon.
Review our best practices when preparing amplicon DNA for PacBio sequencing.
Applications
Features & Benefits
Workflow
Sample Submission Guidelines
GENEWIZ accepts a wide range of inputs for PacBio amplicon sequencing, including purified and unpurified amplicons, restriction digested material, and plasmids. Additionally, GENEWIZ can prepare an amplicon from a wide range of starting materials, including genomic DNA, cell pellets, and tissue material. For detailed sample submission requirements please visit our Sample Submission Guidelines.
Deliverables
Project deliverables include a data summary report, raw data files in BAM format, and demultiplex CCS reads in FASTQ format. Customizable data analysis packages are available by request.
TECHNICAL RESOURCES
Blog: PacBio HiFi Sequencing for Understanding Human Health and Disease
At the Azenta Life Sciences and PacBio virtual symposium titled, Decoding the Complexity of Human Health – A HiFi Vision, speakers from leading research institutions and biotech discussed the role of high-fidelity, long-read sequencing in understanding human health and diseases. In this blog post, we recap the presentations and highlight the themes that emerged from the virtual event.
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