GENEWIZユニバーサルプライマー
PreMixedサービスでユニバーサルプライマーをご利用いただく際には、サンプル提出ガイドライン に準じ、DNAの種類およびDNAのサイズに応じた量のDNAテンプレートをご用意いただき、全量を10uL/wellとしてご提出ください。
| GENEWIZ Legacy Primers | |||
|---|---|---|---|
| Name | Length(nt) | Sequences(5′-3′) | |
|
T7 |
20 |
TAATACGACTCACTATAGGG |
|
|
T7 Term |
19 |
GCTAGTTATTGCTCAGCGG |
|
|
T7 EEV |
Retired |
22 |
ATGTCGTAATAACCCCGCCCCG |
|
T3 |
20 |
ATTAACCCTCACTAAAGGGA |
|
|
SP6 |
19 |
GATTTAGGTGACACTATAG |
|
|
BGHR |
18 |
TAGAAGGCACAGTCGAGG |
|
|
M13F(-21) |
Redesigned1 |
18 |
TGTAAAACGACGGCCAGT |
|
M13F(-47) |
Redesigned2 |
24 |
CGCCAGGGTTTTCCCAGTCACGAC |
|
M13F(-41) |
Redesigned2 |
18 |
GGTTTTCCCAGTCACGAC |
|
M13R |
17 |
CAGGAAACAGCTATGAC |
|
|
CMV-Forward |
21 |
CGCAAATGGGCGGTAGGCGTG |
|
|
5GEX |
23 |
GGGCTGGCAAGCCACGTTTGGTG |
|
|
3GEX |
23 |
CCGGGAGCTGCATGTGTCAGAGG |
|
|
pFastBacF |
20 |
GGATTATTCATACCGTCCCA |
|
|
pFastBacR |
20 |
CAAATGTGGTATGGCTGATT |
|
|
pBAD Forward |
20 |
ATGCCATAGCATTTTTATCC |
|
|
pBAD Reverse |
18 |
GATTTAATCTGTATCAGG |
|
|
EGFP-C |
Renamed* |
22 |
CATGGTCCTGCTGGAGTTCGTG |
|
EGFP-N |
21 |
CGTCGCCGTCCAGCTCGACCA |
|
|
SV40pA-R |
20 |
GAAATTTGTGATGCTATTGC |
|
|
SV40-promoter |
Retired |
20 |
TATTTATGCAGAGGCCGAGG |
|
V5 |
Retired |
21 |
ACCGAGGAGAGGGTTAGGGAT |
|
Myc |
Retired |
24 |
CATGGTCCTGCTGGAGTTCGTG |
|
Neo-F |
Retired |
20 |
CGTTGGCTACCCGTGATATT |
|
Neo-R |
Retired |
20 |
TGGATACTTTCTCGGCAG |
| Updated GENEWIZ Universal Primers | |||
|---|---|---|---|
| Name | Length(nt) | Sequences(5′-3′) | |
|
T7 |
20 |
TAATACGACTCACTATAGGG |
|
|
T7 Term |
19 |
GCTAGTTATTGCTCAGCGG |
|
|
T3 |
20 |
ATTAACCCTCACTAAAGGGA |
|
|
SP6 |
19 |
GATTTAGGTGACACTATAG |
|
|
U6 |
New! |
20 |
GACTATCATATGCTTACCGT |
|
BGHR |
18 |
TAGAAGGCACAGTCGAGG |
|
|
M13F |
Redesigned1 |
16 |
GTAAAACGACGGCCAG |
|
M13R |
17 |
CAGGAAACAGCTATGAC |
|
|
M13-40FOR |
Redesigned2 |
17 |
GTTTTCCCAGTCACGAC |
|
M13-48REV |
New! |
20 |
CGGATAACAATTTCACACAG |
|
CMV-Forward |
21 |
CGCAAATGGGCGGTAGGCGTG |
|
|
5GEX |
23 |
GGGCTGGCAAGCCACGTTTGGTG |
|
|
3GEX |
23 |
CCGGGAGCTGCATGTGTCAGAGG |
|
|
pFastBacF |
20 |
GGATTATTCATACCGTCCCA |
|
|
pFastBacR |
20 |
CAAATGTGGTATGGCTGATT |
|
|
pBAD Forward |
20 |
ATGCCATAGCATTTTTATCC |
|
|
pBAD Reverse |
18 |
GATTTAATCTGTATCAGG |
|
|
EGFP-C-For |
Renamed* |
22 |
CATGGTCCTGCTGGAGTTCGTG |
|
EGFP-C-REV |
New! |
17 |
GTTCAGGGGGAGGTGTG |
|
EGFP-N |
21 |
CGTCGCCGTCCAGCTCGACCA |
|
|
SV40pA-R |
20 |
GAAATTTGTGATGCTATTGC |
|
|
ATTB1 |
New! |
20 |
GTTTGTACAAAAAAGCAGGC |
|
ATTB2 |
New! |
24 |
CCACTTTGTACAAGAAAGCTGGGT |
|
ATTL1 |
New! |
24 |
CGCGTTAACGCTAGCATGGATCTC |
|
ATTL2 |
New! |
20 |
CATCAGAGATTTTGAGACAC |
|
ITS1 |
New! |
19 |
TCCGTAGGTGAACCTGCGG |
|
ITS4 |
New! |
20 |
TCCTCCGCTTATTGATATGC |
|
PJET1-2F |
New! |
23 |
CGACTCACTATAGGGAGAGCGGC |
|
PJET1-2R |
New! |
24 |
AAGAACATCGATTTTCCATGGCAG |
* EGFP-C was renamed EGFP-C-For
1 M13F(-21) was redesigned and renamed M13F
2 Overlapping M13F(-47) and M13F(-41) were reduced to a single primer named M13-40FOR